雌激素受体XbaI和PvuⅡ基因多态性与侵袭性牙周炎的相关研究

Association of estrogen receptor gene XbaI and PvuⅡ polymorphisms with aggressive periodontitis

目的:探讨侵袭性牙周炎(aggressive periodontitis,AgP)易感性与雌激素受体XbaI和PvuⅡ基因多态性的关系。方法:收集48例侵袭性牙周炎(AgP)患者和60例牙周健康对照组的颊黏膜拭子,采用Chelex-100法提取DNA,应用聚合酶链式反应-限制性片段长度多态性技术检测雌激素受体XbaI和PvuⅡ的等位基因及基因型频率在各组之间分布的差异。结果:XbaI基因型分布在AgP组与对照组、AgP组女性与对照组女性、AgP组男性与对照组男性之间均有显著性差异(P<0.05)。其等位基因频率在女性组间有统计学意义(P<0.05)。PvuⅡ基因型分布在AgP组与对照组之间均无明显差异,其等位基因频率分布有明显差异(P<0.05)。多因素logistic回归分析显示男性患病的可能性小(OR=0.352);随年龄增大患病的可能性变小(OR=0.950);当其他条件一定时,xx、Xx基因型的人患病的可能性小(OR值分别为0.678,0.224)。结论:侵袭性牙周炎易感性与雌激素受体XbaI基因型分布有关,XX基因型最易患病,其次为xx,Xx基因型。

Objective： To study estrogen receptor gene Xbal and Pvu Ⅱ polymorphisms in patients with aggressive periodontitis （AgP）. Methods： XbaI and Pvu Ⅱ DNA was extracted by Chelex- 100 and amplified by PCR from buccal swabs of 48 eases of AgP patients and 60 normal controls. The PCR products were analyzed by polymerase chain reaction linked fragment length polymorphism （ PCR-RFLP） assay. Results： There were significant dift）rences of the distribution of Xba I genotype between AgP group and control group, female AgP group and female control group, male AgP group and male control group（ P 〈 0.05 ）. There was no difference of Pvu Ⅱ genolype distribution between patient group and control group（ P 〉 0.05 ）. Multivariate Logistic Regression Analysis showed that male group was less susceptible to AgP than female group（ OR = 0. 352） , the older was less susceptible to AgP than the younger（ Ofl =0. 950）, and the xxXx genotype was less susceptible to AgP than XX genotype [ OR （Xx） =0. 224, OR（xx） = 0. 678 ）. Conclusion： Specific relationship is found between the susceptibility of AgP and the ER gene-Xbal polymorphism. People with XX genotype is more susceptive to AgP than xx, Xx genotypes.