C-反应蛋白基因多态性与慢性牙周炎伴Ⅱ型糖尿病易感性相关研究

Correlation between the C-reactive Protein gene polymorphism in periodontitis and the chronic periodontitis with type2 diabetes

目的:初步分析CRP基因多态性与慢性牙周炎伴Ⅱ型糖尿病易感性之间的关系。方法:收集慢性牙周炎伴Ⅱ型糖尿病患者及慢性牙周炎患者的颊黏膜拭子,采用Chelex-100法提取DNA,然后应用PCR-RFLP法检测CRP的基因型分布。并与牙周健康的对照组基因型对比,统计分析慢性牙周炎及Ⅱ型糖尿病的发病是否与CRP+1059G/C的基因多态性有关。结果:慢性牙周炎伴Ⅱ型糖尿病组、慢性牙周炎组与对照组的CRP+1059基因型分布差异无显著性(χ2=0.223,P=0.994),等位基因频率的分布差异无显著性。3组受试者中GG基因型最多,GC型次之,CC型最少。各基因型及等位基因频率分布均符合Hardy-Weinberg平衡。结论:本研究未发现中、重度慢性牙周炎,慢性牙周炎伴Ⅱ型糖尿病的易感性与CRP+1059G/C单核苷酸多态性有关。

Objective： To study the relationship between C-reactive prntein （CRP） gene polymorphisms and the risk of chronic periodontitis and severe chronic periodontilis（CP） with type2 diabetes to confirm the effect of genetic factor in chronic periodontitis and ehronie periodontitis with type2 diabetes. Methods： DNA was extracted by Chelex-100 from buccal swabs of patients who suffered from chronic periodontitis or chrome periodontitis with type2 diabetes and patients with healthy periodontium. PCR-RFLP was used to test the ClIP genotype distribution, The correlationship between the incidenee of chronic periodontitis and chronic periodontitis with type2 diabetes and CRP gene polymorphism was analyzed statistically, Results： There was no statistical difference in the distribution of CPR ＋ 1059 genotype and allele frequency between experiment group and coutrol group（x^2 = 0. 223, P = 0. 994）. The genotype and allele frequency distribution were in llne with Hardy-Weinberg equilibrium. Conclusion： There is no correlation between CRP＋ 1059G/C single nueleotide polymorphisms and the susceptibility of chronic periodontitis as well as chronic periodontitis with type2 diabetes.