局部振动对雄性兔阴茎勃起功能的影响

Effects of behind legs vibrations on erectile functon in male rabbits

目的通过对雄性兔血清中生殖内分泌激素和阴茎组织中一氧化氮合酶（nitric oxide synthase，eNOS）、内皮素-1（endothelin-1，ET-1）mRNA表达，探讨局部振动对雄性兔勃起功能的影响。方法将新两兰兔随机分为A组（接振强度为3．02m／s^2）、B组（接振强度为6．13m／s^2）、C组（接振强度为12．26m／s^2）和1个对照组，分别在新西兰兔接振试验前和接振试验第10、20和30天，应用双抗体夹心ELISA法检测血清中睾酮、黄体生成素和雌二醇的浓度．于接振试验第30天采用荧光定量PCR法检测阴茎组织巾eNOS、ET-1mRNA表达。结果（1）与接振试验前和对照组比较，A、B、C3个试验组兔血清中睾酮和黄体生成素浓度随接振时间的延长和接振强度的增加呈降低趋势，C组在接振试验第30天血清中睾酮[（1．415±0．713）μg／L]明屁降低，与对照组及接振前比较，差异有统计学意义（P〈0．05）；血清中雌二醇浓度随接振时的间延长和接振强度的增加呈增高的趋势。（2）与对照组相比较，A、B、C3个试验组接振30d后，阴茎组织中ET-1mRNA[B组、C组分别为（17．39±4．59）×10^4、（36.21±13．13）×10^4拷贝数／μg]表达明显增强，eNOS mRNA[A组、B组、C组分别为（19．11±6．83）×10^4、（11．86±3．15）×10^4、（4．68±3．26）×10^4拷贝数／μg]表达明显降低，与对照组[分别为（7．58±3．07）×10^4、（34．88±10．03）×10^4拷贝数／μg]比较，差异有统计学意义（P〈0．01）。结论局部振动可引起血清中睾酮、黄体生成素浓度下降，雌二醇的浓度上升，局部振动通过上调阴茎组织中ET-1mRNA和下调eNOSmRNA的表达，使组织中ET-1合成、释放增加，一氧化氮生成减少，从而影响勃起功能。

Objective To study the effects of behind legs vibrations on erectile function in male rabbits through the concentration of plasma reproductive hormone and the expression of nitric oxide synthase （eNOS）, endothelin-1 （ET-1） mRNA in vibrated male rabbits. Methods 30 male adult rabbits were assigned randomly to A group （vibration power：3.02 m/s^2）,B group（vibration power：6.13 m/s^2）,C group（vibration power： 12.26 m/s^2） and control group.The concentration of expression of eNOS,ET-1 mRNA were measured with RT-PCR after rated for 30 days. Results （1） Compared with 0 days vibration,after expoured to vibration for 10,20,30 days,the A,B,C group concentration of plasma T, LH are much lower（P〈0.05）,the concentration of plasma E2 is much higher. （2） Compared with control group after exposed for 30 days ,the expression of ET-1 mRNA [B group： （ 17.39±4.59）×10^4;C group： （36.21±13.13）×10^4 ] were much higher and expression of eNOS mRNA [A group： （19.11±6.83）×10^4;B group： （11.86±3.15）×10^4：C group： （4.68±3.26）×10^4] was much lower,there were significant differences （P〈0.01）. Conclusions The vibration of behind legs in rabbits resulted the concentration of plasma T,LH are much lower,the concentration of plasma E2 is much higher,increased the expression of eNOS mRNA, decreased the expression of eNOS mRNA,then vary the erectile function.