人髓样细胞触发性受体-1在急性梗阻性化脓性胆管炎外周血单核细胞中的表达

Expression of human triggering receptor expressed on myeloid cells 1 in peripheral blood mononuclear cells of patients with acute obstructive suppurative cholangitis

目的研究髓样细胞触发性受体(TREM)-1在急性梗阻性化脓性胆管炎(AOSC)患者外周血单核细胞(PBMC)中表达的规律及其临床意义。方法分别收集36例AOSC患者(实验组)和40例正常人(对照组)外周血单核细胞,以半定量RT-PCR检测单核细胞中TREM-1 mRNA表达;免疫细胞化学检测单核细胞中TREM-1蛋白表达;ELISA检测患者外周血中TNF-表达水平,免疫透射比浊法测C反应蛋白。结果对照组和实验组TREM-1 mRNA半定量比值分别为0.457±0.053和1.007±0.252(P<0.05)。免疫细胞化学观察结果示实验组TREM-1蛋白阳性表达率与对照组之间差异显著(P<0.01)。实验组TNF-和C反应蛋白水平均高于对照组(P<0.01)。结论人TREM-1在急性梗阻性化脓性胆管炎发病初表达明显升高,提示TREM-1在急性梗阻性化脓性胆管炎的发生中可能起重要作用。

Objective To investigate the expression pattern of human triggering receptor expressed on myeloid cells 1（TREM-1） mRNA in peripheral blood mononuclear cells and its clinical significance in acute obstructive suppurative cholangitis（AOSC）.Methods Peripheral blood mononuclear cells were collected from 36 patients with AOSC and 40 healthy adults.TREM-1 mRNA was determined by semi-quantitative RT-PCR,and TREM-1 protein by immunocytochemistry.Enzyme-linked immunosorbent assay（TNF-α） was used to detect the level of tumor necrosis factor-α（TNF-α）,and immunoturbidimetry employed to detect C reactive protein.Results The expression of TREM-1 mRNA relative to β-actin was 1.007±0.252 in patients with AOSC,significantly higher than that in the healthy adults（0.457±0.053,P〈0.05）.The two groups also showed significantly different TREM protein expression（P〈0.01）.The AOSC patients exhibited significantly higher levels of TNF-α and C reactive protein than the healthy adults（P〈0.01）.Conclusion The expression of human TREM-1 in peripheral blood mononuclear cells is up-regulated obviously in early stage of AOSC,probably suggesting an important role of TREM-1 in the development of AOSC.