不同方法诱导的骨髓基质细胞用于修复山羊髁突软骨缺失的实验研究

Temporomandibular condylar reconstruction with tissue engineered cartilage by different methods:an experimental study on goats

目的:比较用诱导因子诱导及用部分成体软骨细胞诱导的骨髓基质细胞(BMSCs)复合生物材料修复山羊颞下颌关节髁突软骨全层缺失的效果。方法:取山羊6只,分为2个实验组及1个对照组,每组2只。实验Ⅰ组植入经诱导因子(TGF-β1、IGF-1、地塞米松)诱导的BMSCs与支架(Pluronic F-127溶胶)复合物,实验Ⅱ组植入软骨细胞-BMSCs(按3∶7比例混合)的细胞-支架复合物,对照组仅植入支架材料。于术后8周取材,进行颞下颌关节软骨面缺失修复的大体观察、修复组织的HE染色、Ⅱ型胶原分泌的免疫组化染色。结果:术后8周实验Ⅱ组髁突软骨缺失区由软骨样组织修复。HE染色及免疫组化染色结果均提示修复组织为成熟的软骨组织。而实验Ⅰ组及材料对照组缺损区仅由少量的纤维性组织修复,不能形成成熟的软骨组织。结论:骨髓基质细胞在自体软骨细胞基质的诱导下,可以修复山羊髁突软骨面全层缺失,二维培养诱导后的BMSCs在山羊髁突软骨缺失区难以达到修复作用。

PURPOSE： To repair the condylar cartilaginous defects by the differentiated bone marrow stromal cells （BMSCs） combined with Pluronic F-127. METHODS： 6 goats were randomly divided into 3 groups：2 experimental groups with 2 goats and the control group with 2 goats, respectively. In experimental group Ⅰ,BMSCs differentiated by TGF-β1,IGF-1 and dexamethasone were used.In experimental group Ⅱ,autologous BMSCs combined with chondrocytes were mixed with Pluronie F-127 and implanted into goat temporomandibular joint condylar cartilaginous defects.In control group,Pluronic F-127 gel was implanted into their condylar cartilaginous defects. The goats were sacrificed at 8-week. The reconstructive articular cartilage was evaluated by HE staining and immunostaining of type Ⅱ collagen. RESULTS： HE staining and immunostaining of type Ⅱ collagen showed that a layer of reconstructive articular cartilage in the experimental group Ⅱ, but there were no layer of reconstructive articular cartilage in the control group and experimental group Ⅰ. CONCLUSIONS： BMSCs induced by chondroeytes can be differentiated to ehondrocytes and used to repair the condylar articular cartilaginous defects in goats.