DHPG诱导的大鼠海马脑片癫样放电模型基因表达谱分析

Microarray analysis of DHPG-induced rat hippocampal slice epileptic seizure model

目的研究代谢型谷氨酸受体-Ⅰ(mGluR-Ⅰ)激动剂D-对羟基苯甘氨酸(DHPG)诱导的大鼠离体海马脑片癫样放电模型的基因表达谱。方法以含50μmol DHPG的人工脑脊液持续灌流大鼠离体海马脑片,诱导建立癫样放电模型(DHPG组,n=3)。采用cDNA微阵列分析技术获得DHPG组的基因表达谱,与对照组(n=3)比较筛选出差异表达基因,并进行富集功能分类。结果与对照组比较,DHPG组样本模型分别筛选出的上调基因206个,下调基因489个;其中差异表达达1.5倍的上调基因67个,下调基因86个;2.0倍以上的上调基因6个,0.5倍以下的下调基因25个。富集功能分类结果显示,差异表达基因功能涉及蛋白结合(19个)、分子功能、钙离子结合和核苷酸结合等多方面。结论癫样放电及mGluR-Ⅰ激动剂的作用均涉及多种基因,是一复杂的调控过程。实验为进一步研究提供了依据。

Objective To investigate the gene expression pattern of metabotropic glutamate receptor-Ⅰ（mGluR-Ⅰ）,D-p-hydroxyphenylglycine （DHPG）-induced rat hippocampal slice epileptic seizure model. Methods In vitro rat hippocampal sclice was continously perfused with artificial cerebrospinal fluid containing 50 μmol DHPG,and epileptic seizure model was established （DHPG group,n =3）. cDNA microarray chip was applied to explore the gene expression pattern in DHPG group,the differentially expressed genes were screened in comparison with control group （ n =3）,and functional classification analysis was conducted. Results There were 206 up-regulated genes and 489 down-regulated genes,among which 67 up-regulated genes and 86 down-regulated genes differentially expressed by 1.5 fold,6 up-regulated genes differentially expressed by more than 2.0 folds,and 25 down-regulated genes differentially expressed by less than 0.5 fold. Functional classification analysis revealed that differentially expressed gene function involved in protein binding （19 genes）,molecular function,calcium ion binding and nucleotide binding. Conclusion Epileptic seizure and roles of mGluR-Ⅰagonist may be related to various genes,which is a complicated process. This experiment provides evidences for further researches.