蓝湿革上真菌生长:测量影响皮革质量的方法

Fungal Growth on Wetblue:Methods to Measure Impact on Leather Quality

把几种野生的大家所熟悉的真菌接种到标准蓝湿革上,采用不同的方法测量这些真菌对蓝湿革所造成的损害。所选择的真菌来源于美国标准菌库,与那些鞣制过的蓝湿革所污染的菌种不同。把蓝湿革样本接种上所选择的菌种,同时把未接种菌种的蓝湿革作为空白试验,两者同时放置90d时间。革上生出的野生菌株用DNA序列分析方法进行鉴别。对接种和未接种真菌的蓝湿革进行微观观察,发现两者的粒面层和内部组织结构都没有任何差别,说明两者的胶原结构几乎没有受到菌类的侵害。但多种检测表明,接种真菌的蓝湿革与未接种的相比,脂肪含量大幅度下降。

Wild and known fungal species were selected to inoculate a standardized wetblue leather and various methods were then employed to quantify the concomitant damage associated with mold growth. Fungi were isolated from commercially tanned samples of contaminated wetblue and pure strains were sourced from ATCC cultures. Test cuttings of wetblue leather were inoculated with the selected cultures and incubated along with matched control cuttings for a period of 90 days. Unknown wild strains were later identified using DNA squencing techniques. Microscopic observations of all treated versus control samples did not show any difference in grain structure or skin morphology, indicating little or no breakdown of intact and tanned collagen structures. Various staining and analytical assay techniques indicated significant reduction in fatty material content in the treated samples compared with the untreated controls.