雪旺细胞源神经营养因子的神经营养活性及其抗NO神经毒性作用

STUDIES OF NEUROTROPHIC ACTIVITIES OF SCHWANN CELL DERIVED NEUROTROPHIC FACTOR AND ITS RESISTANT EFFECT ON NITRIC OXIDE NEUROTOXICITY

目的：研究雪旺细胞源神经营养因子对脊髓前角神经元的神经营养活性及对抗一氧化氮（ＮＯ）神经细胞毒性的作用。方法：分４组进行神经元培养：（１）营养因子组：加入不同浓度的雪旺细胞源神经营养因子；（２）ＮＯ组：加入ＮＯ体外供体硝普钠；（３）营养因子＋ＮＯ组：同时加入雪旺细胞源神经营养因子和硝普钠：（４）对照组：加Ｄ－Ｈａｎｋ，培养２天后，ＭＴＴ法观察各组细胞情况。结果：营养因子组ＯＤ值均明显高于对照组；ＮＯ组ＯＤ值明显低于对照组：营养因子＋ＮＯ组，当神经营养因子浓度大于３０ｎｇ·孔－１时，ＯＤ值显著高于ＮＯ组。结论：ＮＯ抑制体外培养脊髓前角神经元的存活；雪旺细胞源神经营养因子对体外培养脊髓前角神经元具有明显的神经营养活性，且能对抗ＮＯ的神经细胞毒性。

Objective: To study the neurotrophic activity of SDNF to cultured spinal anterior neurons (SAN )and its resistant effect on NO neurotoxicity in vitro. Method :The dissociated and purified SAN were cultured and divided into 4 groups : ( 1 )SDNF group : SDNF of different density were added. ( 2)NO group :SNP,the provider of NO in vitro, was added. (3)SDNF +NO group:SDNF of different density and SNP were added mean while. (4 )Control group:D-Hank's was added. The cultures were in- cubated for 2 days ,the number of surviving SAN was determined with MTT microassay. Result:In SD- NF group,all the OD values were statistically higher than that of control group. In NO group ,the OD values were statistically smaller than that of control. In SDNF+NO group ,when the density of SDNF was more than 20 ng/well ,the OD values were statistically higher than that of NO group. Conclusion : NO inhibits the survival of cultured SAN;and SDNF not only has significantly the neurotrophic activity to cultured SAN,but also can resist the NO neurotoxicity in vitro.