利用功能标记鉴定普通野生稻中的白叶枯病抗性基因

Application of Functional Marker to Identify Genes for Bacterial Blight Resistance in Oryza rufipogon

以9个菲律宾白叶枯病菌小种对供试的9份普通野生稻(Oryza rufipogonGriff.)及1份高感白叶枯病材料IR24进行抗性鉴定,发现IR24对所有的菌株都表现为高感,6份野生稻材料对9个菌株表现全抗,占参试野生稻总数的67%。取自广西玉林的1份材料只感PXO280(P8),海南万宁的1份材料感PXO71(P4),广州高州的1份材料对PXO79、PXO99和PXO339感病,而这几份材料对其余菌株都表现为抗病,说明每份材料至少含有1个抗性基因。利用已克隆的白叶枯病抗性基因xa5、xa13、Xa21和Xa27的功能标记检测,结果表明9份供试普通野生稻中都不含抗性基因xa5、Xa21;5份为显性Xa13纯合体,4份为隐性抗病xa13杂合体;5份为抗病显性Xa27纯合体,3份为隐性xa27纯合体,1份材料中xa27和Xa27都不存在。

： Field resistance of nine accessions of common wild rice （Oryza rufipogon Griff. ） and a highly susceptible vari ety IR24 was evaluated with nine strains of Xanthomonas oryzae pv. oryzae from the Philippines. The result showed that IR24 was highly susceptible to all of the strains and six common wild rice materials resisted total nine strains, accounting for 67% of the total. Two materials from Yulin, Gnangxi Zhuang Autonomous Region and Wanning, Hainan Province were sus- ceptible to the strains PXO280 and PXO71, respectively, and a material from Gaozhou, Guangdong Province was susceptible to the strains PXO79, PXO99 and PXO339, but they were resistant to the other strains. It is concluded that there was at least one resistance gene in every material. PCR detection for the presence of cloned resistance genes with the functional markers for xa5, xa13, Xa21 and Xa27 revealed that there were no xa5 and Xa21 in all of these materials. Four materials contained recessive resistance gene xal3, which were all heterozygous. There were five homozygotes with dominant resist- ance gene Xa27 and three homozygotes harboring the recessive gene xa27 and one material with neither xa27 nor Xa27.