Bcl-2基因在人肺癌组织中的转录表达研究

The expression of Bcl-2 gene in human lung cancer

目的 探讨Bcl 2基因转录表达与肺癌发生、发展的关系。方法 采用Northern印迹杂交方法检测 3 1例肺癌癌组织、癌旁组织、远癌肺组织和 13例肺良性病变组织Bcl 2mRNA表达水平。结果 ( 1)肺癌组织中Bcl 2mRNA表达水平 ( 179.91± 2 5 .0 2 )明显高于远癌肺组织 ( 13 3 .70± 2 0 .45 )和肺良性病变组织 ( 13 2 .5 3± 13 .85 ) (P <0 .0 1) ;( 2 )低分化肺癌组织 ( 193 .0 7± 2 7.98)中Bcl 2表达强度明显高于中～高分化肺癌 ( 113 .46± 2 1.2 0 ) (P <0 .0 5 ) ;( 3 )Bcl 2mRNA表达水平与肺癌组织学类型、P TNM分期无明显关系 (P >0 .0 5 )。结论 肺癌组织中存在Bcl 2基因的过度转录表达 ,它可能参与调控肺癌的发生。

Objective To explore the relationship between the Bcl 2 gene transcript expression and the oncogenesis, and development of lung cancer.Methods The expression of Bcl 2 mRNA was detected in 31 human lung cancer tissues, para cancer tissues, non cancer tissues and 13 benign lesion tissues by Northern blot.Results (1)The expression level of the Bcl 2 mRNA in the cancer tissues was significantly higher than that in the non cancer tissues and benign lesion tissues(P<0.01), but there was no significant difference among the cancer and para cancer tissues,non cancer and benign lesion tissues, para cancer,non cancer and benign lesion tissues either(P>0.05).(2)The Bcl 2 expression in poor differentiated lung cancers was significantly higher than that in moderate well differentiated lung cancers.(3)No significant difference was observed in Bcl 2 expression in different histoclassification of the cancer and P TNM stages of the cancer.Conclusion There is an overexpression of Bcl 2 mRNA in lung cancer tissues, and it may be involved in the oncogenesis and development of lung cancer.