摘要
目的 研究myc家族癌基因与肺癌预后的关系。方法 应用通用引物RT PCR和非变性聚丙烯酰胺凝胶电泳法对 47例原发性肺癌myc家族癌基因异常表达进行分析。结果 myc基因过度表达与肿瘤大小、淋巴结转移及病期有密切关系。随访 1年 ,myc阳性的病例中复发者占 75% (1 8/2 4) ,myc阴性者中复发者占 47% (7/1 5)。在研究基因间相互作用时发现 :myc过度表达同时伴有p53异常者(myc + /p53 + )占 70 % (1 9/2 7) ,其Ⅲ期及复发病例分别占 63 %和 76% ,高于myc - /p53 +者的 2 7%和2 2 %。结论 myc基因过度表达与肺癌预后有一定联系 ,有可能成为判断肺癌预后不良的指标之一。
Objective To evaluate the relationship between myc family oncogene overexpression and the clinical prognosis of lung cancer. Methods A series of 47 primary lung cancer specimens was analyzed for myc family oncogenes by RT PCR nondenature polycrylamide gel electrophoresis. Results There was a positive correlation between overexpression of myc oncogenes and tumor size,lymph node metastasis and stages of lung cancer.18 of 24 patients(75%) with myc overexpression showed relapse, which was higher than that (47%) without myc gene overexpression for one year postoperatively. The results also showed that 19 of 27 cases (70%) with myc gene overexpression had p53 alteration, and that 63% and 76% of these 19 cases was in stage Ⅲ and relapsed respectively, which was higher than that (27% and 22%) of the patients with p53 alteration but no myc gene overexpression. Conclusion myc family gene overexpression may be regarded as one of the prognostic determinants in lung cancer.
