摘要
以柠檬酸渣为主要原料进行固态发酵生产酸性蛋白酶的试验,研究了不同培养基成分及培养条件对产酶的影响,并进行了曲盘扩大试验和酶学性质探索。结果表明:曲盘扩大培养中,适宜的发酵培养基为:柠檬酸渣65%、麸皮2425%、豆饼粉10%、尿素025%、KH2PO405%;在30℃时,培养时间为48h,酸性蛋白酶酶活力可达到4000μ/g。
The trial was done through acid proteinase produced from the citric acid wastes as main raw materials by solid state fermentation. The effects of different medium compositions and fermentation conditions on the production of proteinase were studied. Meanwhile, the enlarged fermentation experiment and the proteinase characteristics were discussed. The results showed that the activity of acid proteinase was 4 000 μ/g when the mediums (i.e 65% citric acid wastes, 24.25% wheat bran, 10% soybean cake meal, 0.25% urea, 0.5% KH 2PO 4) cultivated for 48 hours at the temperature of 30 ℃ in the enlarged fermentation.
出处
《粮食与饲料工业》
CAS
北大核心
1998年第11期25-27,共3页
Cereal & Feed Industry
基金
国家环保局科技发展项目
关键词
柠檬酸渣
固态发酵
酸性蛋白酶
产酶
发酵饲料
citric acid waste solid-state fermentation acid proteinase conditions of producing proteinase enzymic activity