SPE-GC/MS法检测人血中的鸦片类毒品

Analysis of Opiates in Human Blood by Solid-Phase Extraction and Gas Chromatography/Mass Spectrometry

采用混合型强阳离子固相萃取柱（SPE）对血液中的吗啡、可待因、6-单乙酰吗啡3种鸦片类毒品进行提取,提取液吹干后,再用N-甲基-双三氟乙酰胺（MBTFA）衍生化,进行GC/MS-SIM检测。以乙基吗啡为内标,3种毒品检测的线性相关系数均〉0.99,线性范围为10-1 000μg/L,相对回收率分别为90%-116%、90%-110%、79%-102%;日内和日间相对标准偏差分别小于10%和16%;检测限分别为1、0.5和3μg/L。该方法灵敏度高、重现性好、操作简便,可用于鸦片类毒品滥用者或中毒者血液中的毒品及其代谢物的检测。

Morphine,codeine and 6-acetyl morphine in human blood were detemined via solid-phase extra-ction and gas chromatography/mass spectrometry-selected ion monitoring,with ethyl morphine as internal standard,3 opiates spiked in blank blood were extracted wtih a solid-phase cartridge.The fluent was evapo-rated to dryness followed by being derivatized with MBTFA,and then it was analyzed directly by GC/MS in selected ion mode.The method was validated in a range of 10-1 000 μg/L with the drug-spiked blood,and the reactor responded linearly over the studied range（R20.99 for the 3 drugs）.The relative standard deviations（RSD） for intra and inter-days are less than 10% and 16% respectively;the relative extraction recoveries are 90%-116%,90%-110% and 79%-102% sequenfyl for 3 drugs above,and LODs were less than 1,0.5 and 3 μg/L respectively,which promised the perspective application of the method in detecting trace amounts of opium in human blood.