自体CIK细胞治疗恶性肿瘤免疫表型和杀伤活性变化

Difference of immune phenotypes and NK cell activity after treatment of autologous CIK cell on malignant tumor

目的观察自体CIK细胞治疗恶性肿瘤前后免疫袁型和杀伤活性变化。方法采用流式细胞术检测50例肿瘤患者CIK细胞治疗前、后免疫表型的变化，采用3H—TdR释放法检测杀伤活性的变化。结果CIK治疗后，CD3^＋、CD4^＋细胞百分率上升，CD8^＋细胞百分率下调，CD4^＋／CD8^＋比值上升，CD19细胞百分率下降，与治疗前比较差异有统计学意义（P〈0．05），但CD3^＋、CD4^＋、CD8^＋细胞绝对值未达到健康者水平（P〉0．05）。治疗后NK细胞百分率明显升高（P〈0．05），达到健康者水平（P〉0．05）。在杀伤活性的研究中，与CIK细胞治疗前（19±6）％相比，治疗后杀伤活性百分率（22±5）％明显升高，达17．5％（P〈0．01），但未达到健康者水平（P〈0．05）。结论自体CIK细胞治疗能明显提高肿瘤患者细胞免疫功能。

Objective To investigate the difference of immune phenotypes and NK cell activity after treatment of autologous CIK cell on malignant tumor. Methods In the 50 patients with malignant tumor infused with CIK cell, the differences of immune phenotypes and activity of NK cells were respcetitively measured using FACS and 3H - TdR release assay. Results Compared with pre - treatment of autologous CIK cell, CD3^＋ and CD4^＋ expression percentage rise were up -regulated significantly, CD8^＋ cell expression percentage rise decline; The CD4^＋/CD8^＋ ratio rise, CD19 cell expression percentage rise decline, the comparison has signifieanct difference （ P 〈 0.05 ）. After treatment of autologous CIK cell CD3^- CD56^＋ cell expression percentage increase remarkablely. In the study of NK activity, NK activity （ 22 ± 5 ） % , was higher obviously than pre - treatment （ 19 ± 6 ） %. Conclusions Treatment ofautologous CIK cell can enhance recent immunity function of the tumor patients obviously.