去甲斑蝥素对肺癌A549细胞株体外放射增敏作用

Radiosensifizing effect of norcantharidin on lung cancer A549 cell line in vitro

目的研究去甲斑蝥素对肺癌A549细胞株体外放射增敏作用及其可能存在的增敏机制。方法肺癌A549细胞体外常规培养，通过不同的去甲斑蝥素注射液毒性试验，选取合适的浓度进行增敏试验，分组为空白对照组，单纯加药组，单纯照射组，加药照射组，射线剂量采取2Gy和5Gy，采用MTT法测定各组对细胞的抑制率，应用流式细胞仪测定不同浓度药物的诱导细胞周期再分布及其凋亡作用。结果去甲斑蝥酸钠注射液1μg／ml组与空白对照组对比，50μg／ml组与100μg／ml组对比差异无统计学意义（P〉0．05），余各剂量组的OD值之间相比差异有统计学意义（P〈0．05）。照射药物组与单纯照射组、照射药物组与单纯药物组两两比较差异均有统计学意义（P〈0．05）。随着去甲斑蝥酸钠注射液浓度的增加，细胞的G2／M比例及凋亡率呈明显增加趋势，经趋势卡方检验，差异有统计学意义（P〈0．05）。结论去甲斑蝥素具有放射增敏作用，其放射增敏作用的可能机制为：直接抑制作用、增加对放射敏感的G2／M期细胞比例及诱导凋亡作用。

Objective To investigate the radiosensitizing effect of norcantharidin on lung caner A549 cell line in vitro. Methods Lung cancer A549 cells were conventionally cultured. Select the suitable concentration with different concentrations disodium norcantharidinate injections toxicity test. It was divided into 4 groups： blank control group, drug group, radiation group and drug and radiation group. The dose of ray was 2 Gy and 5 Gy. Detect the OD humerus and calculate the inhibition ratio. Detect the cell cycle and apoptosis which induced by different concentrations disodi-um norcantharidinate injections with flow cytometry. Results The difference was statistical significance （ P 〈 0. 05 ） between every experiment group and the control group, the differences between radiation drug group and radiation group, radiation drug group and drug group both were statistical significance （ P 〈0.05）. Conclusions Norcantharidinate could induCe radiosensitizing effect. Direct repression, increasing the ratio of G2/M and inducing apoptosis could be the possible mechanisms.