莫诺苷抑制大鼠脑缺血再灌注模型皮层脂质过氧化作用的研究

Morroniside Inhibiting Lipid Peroxidation of Cortex in Rats with Focal Cerebral Ischemia/Reperfusion

目的研究莫诺苷对大鼠脑缺血再灌注皮层脂质过氧化作用的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,缺血30min,再灌注72h。以维生素E(VE)为阳性对照药,采用分光光度法检测脑组织中丙二醛变化。结果与假手术组相比,模型组丙二醛含量明显增加(P<0.01);与模型组相比,莫诺苷30mg/kg、90mg/kg能够降低丙二醛含量(P<0.05),莫诺苷270mg/kg能明显降低丙二醛含量(P<0.01);VE(35mg/kg)能显著减少丙二醛的含量(P<0.001)。结论莫诺苷能通过抑制皮层脂质过氧化水平而发挥神经保护作用。

Objective To investigate the effects of morroniside on lipid peroxidation of cortex in rats with focal cerebral ischemia/reperfusion. Methods The animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. The content of malondialdehyde （MDA） was detected with spectrophotometry. Results Compared with sham group, the content of MDA increased obviously in model rat. Compared with model group, Morroniside（30 mg/kg, 90 mg/kg,270 mg/kg）and vitamin E（35 mg/kg） decreased the content of MDA significantly （P〈0.05）. Conclusion Morroniside may protect the cortex from lipid peroxidation.