2(3)-叔丁基-4-羟基茴香醚对阿霉素诱发小鼠毒性的保护作用及其抗氧化机制

PROTECTIVE AND ANTIOXIDATIVE EFFECT OF 2(3)TERT BUTYL 4 HYDROXYANISOLE AGAINST CYTOTOXICITY INDUCED BY DOXORUBICIN IN MICE

探讨叔丁基羟基茴香醚（ＢＨＡ）对阿霉素诱发小鼠毒性的保护作用及其机制。测定一些酶的活性及ＭＤＡ含量，并计数小鼠死亡率。结果经ＢＨＡ预处理后小鼠血清ＧＰＴ，ＧＯＴ，ＬＤＨ和ＣＫ活性及死亡率与阿霉素组比较均显著降低，并能显著降低由阿霉素所致ＭＤＡ含量升高的作用，同时ＢＨＡ对心肌及肝组织醌还原酶、ＧＳＴｓ和谷胱甘肽还原酶活性诱导增高。提示ＢＨＡ对阿霉素的毒性具有良好的保护作用。

The protective and antioxidative effects of 2(3)tert butyl 4 hydroxyanisole (BHA) against cardiotoxicity and hepatotoxicity induced by doxorubicin in mice were investigated. After pretreatment with different oral doses of BHA, doxorubicin 30 mg·kg -1 was given ip. Serum GPT, GOT, LDH and CK were determined, and the mortality rate of animals was observed. Quinone reductase (QR), glutathione S transferases (GSTs) and glutathione reductase (GR) were determined on tissue cytosols with enzyme dynamic methods. Malondialdehyde (MDA) was measured by the method of thiobarbituric acid. Compared with the doxorubicin group, the serum GPT, GOT, LDH, CK and the mortality rate of mice were significantly decreased by BHA pretreatment, and BHA was shown to inhibit the increase of MDA induced by doxorubicin ( P <0 01 and P <0 0001). Administration of BHA resulted in increased activities of QR, GSTs and GR in the myocardium and liver ( P <0 05 or P <0 0001). These results suggest that BHA has protective effect against the toxicity induced by doxorubicin via the induction of QR, GSTs and GR activities and anti lipid peroxidation.