曼氏裂头蚴感染小鼠外周血T淋巴细胞亚群及IL-4、IL-10、IFN-γ、TNF-α的动态变化

Changes in peripheral blood T lymphocytes,IFN-γ,TNF-α,IL-4,and IL-10 in mice infected with Spargana

目的观察小鼠感染裂头蚴后外周血T淋巴细胞亚群及细胞因子含量的动态变化,了解小鼠感染后的细胞免疫特点。方法从黑斑蛙体内检获裂头蚴,口服法感染昆明小鼠,5条/只。感染后第2～10周分批处死小鼠,用流式细胞仪检测外周血T淋巴细胞亚群(CD4+T、CD8+T),用双抗夹心ELISA法检测血清IFN-γ、TNF-α、IL-4、IL-10的含量变化。结果实验鼠CD4+T细胞百分比在感染后第2～5周呈上升趋势,之后逐渐下降,第9周降至(31.61±5.33)%,第10周又回升到第8周水平;CD8+T细胞百分比升高,第4周达高峰,为(41.69±15.84)%。IFN-γ在感染后第3周为(13.36±2.58)pg/ml,第4周为(13.96±4.53)pg/ml,第5周为(13.33±2.32)pg/ml,与对照组相比差异有统计学意义(P<0.05);TNF-α第4周达(22.99±4.52)pg/ml,之后呈下降趋势,第10周降至(10.99±1.14)pg/ml;IL-4第4周开始上升,第7周达(18.31±7.70)pg/ml,第8周后逐渐下降;IL-10从第5周起逐渐上升,至第8周达到(15.89±4.64)pg/ml,之后呈下降趋势。结论小鼠感染裂头蚴早期,发生Th1免疫应答;随着感染的进程,免疫应答向Th2偏移。Th1/Th2型免疫应答发生的时相和效应强度可能影响裂头蚴感染的最终结局。

Objective To observe the changes in peripheral blood T lymphocytes,IFN-γ,TNF-α,IL-4,and IL-10 in mice infected with Sparganum.Methods The 135 mice were infected orally with Spargana collected from Rana nigromaculata（5 strips/mouse）.Peripheral blood cells and serum were collected.The level of CD4^＋,CD8^＋,IFN-γ,TNF-α,IL-4,or IL-10 was analyzed by flow cytometry and ELISA respectively at 2-10 weeks post-infection.Results The level of CD4^＋ T cells increased constantly from 2 to 5 weeks and then was significantly lower than that in the control group and recovered in week 10. The level of CD8^＋ T cells was higher than that in the control group and peaked in week 4（41.69± 15.84）. The level of IFN-γ was higher than that in the control group in weeks 3 10 and peaked in week 4（13. 96±4.53）. The level of TNF-α, IL-4, and IL-10 peaked in week 4, week 7, and week 8, respectively. Conclusion Cellular immunity plays an important role in resisting infection with Spargana. CD8^＋ T cells are important immune cells. Aceording to dynamic cytokine detection, the Thl immune response was dominant in the early infection when mice were infected with Spargana. Then, it changed to Th2 response. The shifting of Th1/Th2 immune response had an effect on the final outcome of Sparganum infection.