四倍体药蒲公英和二倍体山西多裂蒲公英中咖啡酸含量的比较

Comparison of caffeic acid contents in tetraploidy taraxacum officinale and diplontic taraxacum dissectum

本文采用四倍体药蒲公英和二倍体多裂蒲公英为材料,通过HPLC法测定并比较两种蒲公英中咖啡酸的含量,以大力推广产量高,抗逆性强,药用活性成分高的优质多倍体蒲公英新品种。HPLC所采用的色谱柱为ApolloC18柱(150mm×4.6mm,5μm),流动相为甲醇-磷酸盐缓冲液(pH4.0)(23?77);柱温40℃;流速0.9mL/min,检测波长为327nm。二倍体山西多裂蒲公英中咖啡酸含量仅为0.105%,四倍体药蒲公英中咖啡酸含量高达0.29%,比前者多176.1%。四倍体药蒲公英中咖啡酸含量高,是一种值得推广的优质多倍体蒲公英新品种。

A high performance liquid chromatographic method was established for determination of caffeic acid contents in tetraploidy taraxacum officinale and diplontic taraxacum dissectum. An Appollo C18 column （150mm×4.6 mm, 5μ） was used with CH3OH-PB （pH4.0, 23：77） as mobile phase, detection at 327nm, column temperature of 40℃, and flow rate of 0.9mL/min. The results showed that the caffeic acid content in tetraploidy taraxacum officinale was 0.29 %, and in diplontic taraxacam disseetum it was only 0. 105%. The former was 176.1% higher than the latter. The comparison is helpful for promoting high quality tetraploidy strain.