瘢痕疙瘩不同部位Ⅰ、Ⅲ型前胶原mRNA的原位表达研究

Study on in situ expression of type Ⅰ and Ⅲ procollagen mRNAs in different parts of keloid and normal skin

目的基于瘢痕疙瘩浸润、增生和老化部组织形态学上的差异,为了明确瘢痕疙瘩不同部位及正常皮肤Ⅰ、Ⅱ型前胶原mRNA的表达是否存在差异。方法对4例瘢痕疙瘩和2例正常皮肤通过原位杂交方法,观察了Ⅰ、Ⅱ型前胶原mRNA的表达。结果瘢痕疙瘩的Ⅰ、Ⅱ型前胶原mRNA的表达比正常皮肤明显增强,尤以Ⅰ型为甚,导致Ⅰ/Ⅱ型前胶原的比率增高,且其表达在瘢痕疙瘩浅层多于深层,浸润及增生部多于老化部,浸润和增生部的表达无明显差别。结论瘢痕疙瘩的不同病理部位和正常皮肤Ⅰ、Ⅱ型前胶原mRNA的表达存在差异,是形成瘢痕疙瘩不同病理部位的原因之一。

Objective Based on the histomorphologic differences of three parts of keloid, i, e., in- vasive, proliferative and senile regions ,the study was carried out to explore the differences of expression of type Ⅰ and type Ⅲ procollagen mRNAs in the three different parts of keloid and normal skin. Method Four samples of keloid from four patients and two samples of normal skin were collected. The expression of pro_(αl) ( Ⅰ) and pro_(αl) (Ⅲ ) procollagen mRNAs in keloid and normal skin was investigated by in situ hybridization techniques. Result Both type Ⅰ and Ⅲ procollagen mRNAs levels increased in keloid, especially type Ⅰ,resulting in elevated ratios of type Ⅰ to Ⅲ procollagen mRNA. In keloid, ex- pressive intention of type Ⅰ and type Ⅲ procollagen mRNAs was higher in the papillary layer than in the reticular layer. Also the expressive intention of type Ⅰand type Ⅲ in keloid was higher in invasive and proliferative regions than in the senile region. However, expressive intention of type Ⅰ and type Ⅲ procollagen mRNAs in the invasive region of keloid was similar to that in the proliferative region. Con- clusion The differences of expression of type Ⅰ and type Ⅲ procollagen mRNAs in different pathologi- cal regions of keloid and normal skin is one cause of forming different pathological regions in keloid.