内皮素-1与脑血管痉挛缺血性脑损害的关系及银杏叶制剂的保护作用

Relationship between Endothelin-1 and Ischemic Brain Damage after Subarachnoid Hemorrhage and Protective Effect of Ginkgo Biloba Extract

目的 :探讨内皮素 1 (endothelin 1 ,ET 1 )与蛛网膜下腔出血 (subarachnoidhem orrhage,SAH)后缺血性脑损害的关系和银杏叶制剂 (ginkgobilobaextract,GBE)的防治作用。方法 :应用非开颅大鼠模型 ,对SAH组和GBE组测量基底动脉 (basilarartery ,BA)管径并观察2 4h内微区脑血流量 (regionalcerebralbloodflow ,rCBF)和颅内血浆ET 1水平动态改变 ,3天后对海马CA1区行病理检查。结果 :SAH后rCBF迅速而持续降低 ,血浆ET 1浓度显著增加 ,BA痉挛 ,海马CA1区神经元明显受损。GBE使上述改变均减轻。结论 :SAH时ET 1增加是导致缺血性脑损害的重要因素 ,GBE通过拮抗ET 1病理性增多而减轻缺血性脑损害。

Objective: To investigate the role of endothelin 1 (ET 1) in development of ischemic brain damage after subarachnoid hemorrhage (SAH), and the protective effect of Ginkgo biloba extract (GBE). Methods: Wistar rat noncraniotomy models of SAH were divided into SAH group and GBE treated group, the diameter of basilar artery (BA) and dynamic changes of regional cerebral blood flow (rCBF) and ET 1 content of intracranial plasma within 24 hours after SAH of both groups were determined. And pathological examination of CA1 region of hippocampus was performed 3 days later. Results: rCBF decreased and ET 1 content increased obviously and retained in 24 hours after SAH. Spasm of BA occurred half an hour after SAH and neurons of hippocampus CA1 region was damaged severely. GBE could antagonize the above mentioned pathological changes effectively. Conclusion: Increase of ET 1 is an important factor leading to ischemic brain damage after SAH. GBE exerts its protective effect by antagonizing pathological increase of ET 1.