比格犬血浆中托烷司琼的HPLC法测定

Determination of Tropisetron in Beagle Dogs' Plasma by HPLC

建立了比格犬血浆样品中托烷司琼的反相高效液相色谱检测法。血浆样品 ,加入盐酸三环哌酯作内标 ,用乙酸乙酯提取 ,经稀盐酸反洗 ,再用二氯甲烷提取。色谱条件 :C8柱 ;流动相 :甲醇 -水 (5 5∶4 5 ) ,加 0 3%三乙胺 ,用冰醋酸调pH 7 2 ;检测波长 2 83nm。线性范围 5 4 5～70 0ng/mL ,最低检测浓度为 3 0ng/mL ,回收率为 79 9%～ 85 8% ,日内RSD为 1 0 6 %～4 2 7% ,日间RSD为 2 94 %～ 9 89%。采用本法对比格犬口服托烷司琼的药代动力学进行了研究。

A reversed-phase high performance liquid chromatographic method is described for the determination of tropisetron in plasma of beagle dogs.Tropisetron in plasma was extracted with ethyl acetate after the addition of the internal standard tricyclopinate hydrochloride.The organic phase was re-extracted with dilute hydrochloric acid and the aqueous phase was back-extracted with dichloromethane.After the extract was evaporated to dryness,the residue was dissolved in methanol and then separated on a C 8 column.The mobile phase consisted of methanol-water(55∶45)with 0 3 % triethylamine and was adjusted to pH 7 2 by acetic acid.The detector was set at UV 283 nm.The linear range of the calibration curve was 5 45～700 ng/mL and the minimal detectable concentration was 3 0 ng/mL.The recovery of the method was 79 9 %～85 8 %.The within-day and between-day precisions were 1 06 %～4 27 % and 2 94 %～9 89 %,respectively.The method has been applied to the pharmacokinetic study of tropisetron in beagle dogs after po 20 mg.fbeagledogs .Tropisetronin