气相色谱内标法测定蚕蛹油中α-亚麻酸含量

Content Determination of α-Linolenic Acid in Can Yong Oil by GC Internal Standard Method

化学法提取的蚕蛹油经甲酯化后 ,采用气相色谱 /质谱 (GC/MS)技术 ,对蚕蛹油中脂肪酸的组分进行了分析 ,以十九烷酸 (19∶0 )甲酯作内标物 ,气相色谱法 (GC)对蚕蛹油中α -亚麻酸进行了定量测定。结果表明蚕蛹油中α -亚麻酸的含量高达 72 80 %。本研究为α

Can Yong oil was extracted by chemical method.The fatty acid(FA)in Can Yong oil was saponificated by KOH-MeOH and was modified to methyl esters by means of BF 3-MeOH,and was checked with thin-layer chromatography.The compositions of fatty acids were analyzed by GC/MS.The method of GC internal quantification of α-Linolenic in Can Yong was established,using internal standard of C 19∶0 .A linear relationship was obtained in the concentration rage of 0 125～2 000 μg/μL and the correlation coefficient was 0 9830.The content of α-Linolenic acid was 72 80 %.The study may give a test method for determination of α-Linolenic.