黄芩茎段腋芽诱导与植株再生试验

Resarch on Axillary Bud Induction and Plantlet Regeneration in Scatellaria baicalensis Georgi Stem

以黄芩茎段为外植体,在附加不同植物激素组合的培养基中对腋芽的诱导和再生植株进行试验研究。结果表明:MS+6-BA1.5mg/L+IBA 0.2mg/L培养基有利于促进腋芽萌发和生长;最有效的增殖培养基为MS+6-BA 1.0mg/L+IBA 0.5mg/L,幼芽的增殖系数高达11.9;最有效的生根培养基为1/2 MS+IBA 0.5mg/L,生根率可达90%。

The axillary bud and plantlet regeneration were studied on different plant hormone combination media taking Scatellaria baicalensis Georgi stem as explant.The results showed that MS＋6-BA 1.5mg/L＋IBA 0.2mg/L was an appropriate culture medium for germination and growing of axillary buds;the best medium for propagation of buds was MS＋6-BA 1.0mg/L＋IBA 0.5mg/L,the coefficient of propagation was upon to 11.9;the best rooting medium was 1/2 MS＋IBA 0.5mg/L,in which the rooting rate reached 90%.