多重PCR检测FFv三个基因座在景颇族人群中的遗传多态性

Distribution Of F13A01, FESFPS and vWA loci in Jingpo ethnic group in Yunnan provice

短串联重复序列（STR）是由几个碱基对作为核心单位串联重复形成的一类DNA序列，作者将3个STR基因座在同一反应体系中进行互不干扰的复合扩增，采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对云南省景颇族的F13A01，FESFPS和vWA等3个基因座等位基因的基因频率进行了调查，获得了满意的结果，显示了广阔的应用前景。F13A01基因座观察到8个等位基因、13个基因型；FESFPS基因应观察到7个等位基因、18个基因型；vWA基因应观察到7个等位基因、21个基因型。

F13A01 FESFPS and vWA were extracted and amplifiedusing denaturing polyacrylamide gel electrophoresis and silverstain. The distribution of allete frequencies of F13A01, FESFPS and vWA loci in Jingpo ethic group living in southwestern Yunnan province. were investigated. It was observed thatthere were 8 alleles and 13 genotypes in F13A01 locus; 7 alleles and 18 genotypes in FESFPPS locus; 7 alleies and 21genotypeS in vWA lous.