层粘素及其受体在人卵巢癌细胞中的表达和意义

Expression and Significance of Laminin and Its Receptor in Human Ovarian Cancer Cells

目的探讨层粘素(laminin,LN)及其受体(laminin receptor,LNR)在人卵巢癌细胞黏附和转移中的作用。方法采用细胞黏附实验检测层粘素及其受体在5株卵巢癌细胞黏附中的作用;Boyden小室测定癌细胞侵袭能力;流式细胞技术检测层粘素及其受体在人卵巢癌细胞上的表达情况。结果抗层粘素及其受体抗体,可明显抑制人卵巢癌细胞的黏附作用,且随着稀释度的不同,其抗黏附能力各异。抗层粘素抗体侵袭力测定显示,A-2780最弱,Skov-3最强,两者比较,差异有显著意义(P<0.01)。在5种卵巢癌细胞中,4株癌细胞有层粘素及其受体表达,1株癌细胞无表达。结论层粘素及其受体可通过诱导肿瘤细胞黏附和迁移,从而促进肿瘤细胞浸润和转移。

Objective To explore the function of laminin（LN） and laminin receptor （LNR） in the adhesion and metastasis of human ovarian cancer cells. Methods The adhesion function of laminin and its receptor in 5 kinds of ovarian cancerous cells were evaluated by cell adhesion test, and the invasion ability of ovarian cancerous cells was tested by Boyden chamber. Meanwhile, the expression of laminin and its receptor in the ovarian cancerous cells was tested by flow cytometry. Results Anti-laminin and its receptor antibody could significantly inhibited the adhesion function of human ovarian cancer cells, and the anti- adhesion ability of anti-laminin and its receptor antibody varied with the variation of dilution degrees. Boyden chamber test showed that A-2780 had the lowest invasive activity and Skov-3 had the highest invasive activity compared with other three cell lines（P^0.01）. Among 5 kinds of ovarian cancerous cells, 4 of them had the expression of laminin and laminin receptor; the other one did not express laminin and laminin receptor. Conclusion Laminin and its receptor may play a key role in the adhesion and migration of malignant tumor cells so as to promote the invasion and metastasis of tumor cells.