p185抗体可致小鼠流产的研究

Study on miscarriage of mice immunized with p185

目的通过体内实验进一步验证ｐ１８５蛋白的免疫原性，旨在研究该蛋白作为肿瘤疫苗的可能性。方法用ＳｅｐｈａｄｅｘＧ２００层析柱分离的ｐ１８５蛋白组分与佐剂混合免疫ＢＡＬＢ／ｃ小鼠，经ＥＬＩＳＡ验证血清中出现ｐ１８５抗体后与健康的同品系雄性鼠交配，以阴栓形成提示交配成功，观察雌鼠产仔率，子宫形态及免疫病理。结果免疫鼠产仔率明显低于仅用佐剂免疫的对照组（Ｐ＜０．０５），子宫内发现胚胎坏死，病检显示子宫组织有弥散性中性粒细胞浸润和局灶性坏死，并有免疫复合物沉积，提示ｐ１８５免疫能诱导小鼠流产。结论这一实验结果从体内进一步证实ｐ１８５的免疫原性。为该蛋白作为肿瘤疫苗提供实验资料。

Objective To identify the immunogenicity of p185 onco protein in vivo and to study its possibility as a tumor vaccine. Methods p185 HER2/neu onco protein crude products,derived from 3T3 neu transfected cell lysates by Sephadex G200 chromatography column,were used with adjuvant to ip immunize female BALB/c mice.When 185 antibody positive the mice were mated with males.Successful mating was evidenced by the presence of vaginal embolus. Results The average of the offspring per delivery from immunized mice was significantly lower than that from the control mice immunized with adjuvant alone.The pathological findings revealed that there were diffused infiltration of neu trophiles,focal necroses as well as immunocomplex deposits on the wall of uterus resulting in necrotic fetuses.These indicated that the pregnant mice which had been immunized with p185. Conclusions Above results suggest the p185 onco protein are the tumor antigen and can induce the immune response in BALB/c mice.This model may be useful for studying the immunogenicity of the fetal tumor antigen.