基于HPLC-ELSD法的中药复方制剂中黄芪甲苷含量测定

Content Determination of Astragaloside Ⅳ in Chinese Herbal Preparation Based on HPLC-ELSD Method

[目的]利用高效液相-蒸发光散射检测法测定中药复方制剂中黄芪甲苷的含量。[方法]采用Phenomenex公司C18(250.0 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(37∶63),流速为0.85 ml/min,检测器为ELSD 2000。[结果]黄芪甲苷在0.396～3.564μg范围内呈线性,回收率为97.94%(RSD=0.68%)。样品含量为0.288 3～0.299 5 mg/片,RSD为0.36%～1.20%。[结论]该法具有良好的精密度和重现性,结果准确可靠,可作为中药复方制剂的质量控制方法。

[ Objective ] The research aimed to determine the content of astragaloside IV in Chinese herbal preparation by HPLC-ELSD method. [ Method] The determination conditions were as follows ： using C18 （250 mm× 4.6 mm,5μm） chromatographic column of Phenomenex Company, using acetonitrile ： water （37：63） as the mobile phase, flow rate of 0.85 mL/min, using ELSD 2000 detector. [ Result] Astragaloside IV showed the linearity in the range of 0.396 - 3. 564 μg and the recovery rate was 97.94 % （RSD = 0.68% ）. The content of astragaloside IV in samples was 0.288 3 - 0. 299 5 mgper tablet with RSD of 0.36% - 1.20%. [ Conclusion ] This method is accurate and reproducible. It can be used for the quality control of Chinese herbal preparation.