白术茎尖的离体快繁研究

Study on Rapid Propagation of Atractylodes macrocephala koidz.Shoot Tip

[目的]建立白术茎尖的离体快繁体系,筛选最佳培养基。[方法]以白术的茎尖为外植体,设置7种附加NAA、IBA、6-BA不同浓度配比的MS培养基进行茎尖诱导分化与增殖培养,设置5种附加不同浓度NAA的1/2 MS培养基进行生根培养,将驯化后的试管苗置于草木灰与草炭土(1∶2)的混合基质进行移栽试验。[结果]7种诱导分化与增殖培养基中,MS+6-BA0.4 mg/L+NAA0.1 mg/L的培养基有利于白术芽的萌发,芽成活率达100%;MS+6-BA0.5 mg/L+NAA0.1 mg/L的培养基有利于诱导丛生芽增殖。5种生根培养基中,以1/2 MS+NAA0.1 mg/L+活性炭0.5 g/L诱导白术生根的效果最佳,生根率达66.7%。驯化后的试管苗在草木灰和草炭土的混合基质中培养,试管苗成活率高。[结论]该研究为白术实现工厂化育苗及药用次生代谢产物研究提供了试验依据。

[ Objective ] The study aimed to establish the rapid propagation system of Atractylodes macrocephala koidz, shoot tip and screen the optimum medium. [ Method ] With the shoot tip of A. macrocephala koidz, as explant, 7 kinds of MS media added NAA, IBA and 6-BA with different concn, matching were set up to make for the shoot tip culture of induced differentiation and proliferation and 5 kinds of 1/2 MS media added NAA with different concn, were set up to make the rooting culture, and then the domesticated test tube seedlings were cultured in the mixed matrix of plant ash and peat soil （1：2） for transplanting test. [ Result] Among 7 kinds of MS media for induced differentiation and pro- liferation, MS ＋ 6-BA 0.4 mg/L ＋ NAA 0.1 mg/L was conducive to the germination of A. macrocephala koidz, buds, with the bud survival rate of 100% ; MS ＋6-BA 0.5 mg/L ＋ NAA 0.1 mg/L was conducive to the proliferation of induced multiple shoot clumps. Among 5 kinds of rooting media, 1/2 MS ＋ NAA 0. 1 mg/L ＋ activated carbon 0.5 g/L could get optimum effect, with rooting rate of 66.7%. When the domesticated test tube seedlings were cultured in the mixed matrix of plant ash and peat soil for transplanting, the test tube seedlings could get higher survival rate. [ Conclusion ] This study provide the test basis for realizing industrial seedling of A. macrocephala koidz, and studying medicinal secondary metabolites.