贵州兰花SPAD值和叶绿素含量测定与分析

Determination and Analysis of SPAD Value and Chlorophyll Contents in Cymbidium from Guizhou

利用SPAD-502叶绿素检测仪和常规方法对贵州兰花7个种的21个品种叶绿素含量进行了比较分析。结果表明,大多数兰花在种间或种类叶绿素a含量、叶绿素b含量、总叶绿素含量以及SPAD值有所不同,但之间并没有表现出优异的差异性;部分品种的叶绿素含量与其SPAD值之间的相关性未达到显著水平,说明利用SPAD值来衡量其叶绿素含量应慎重;通过聚类分析可以把21个品种分为4类,但大多数叶绿素含量处于相当的水准。

The chlorophyll content in 21 cultivars of 7 Cymbidium species in Guizhou Province were measured and compared by using SPAD- 502 chlorophyll detector and conventional methods. The results showed that the chlorophyll a, chlorophyll b, total chlorophyll and SPAD value of most Cymbidium cuhivars in and between species were different, without significant difference. The relativity of segment cuhivars between the chlorophyll content and SPAD value were not significant, which indicated that the chlorophyll content should be weighed by SPAD value cautiously. Cluster analysis results showed that all these Cymbidium species could be divided into 4 classes, but the chlorophyll content of most cultivars were at certain levels.