硅酸盐细菌DMS3促进钾长石释钾及绿豆生长作用研究

Study on K-release Effect of Silicate Bacterium DMS3 on the Promotion of K-feldspar and the Growth of the Mung Bean

[目的]研究硅酸盐细菌的解钾作用,为利用该菌生产生物钾肥提供科学依据。[方法]从不同地区土样筛选硅酸盐细菌,选出高效菌株DMS3,并进行生理生化鉴定和16S rDNA的PCR分析,对DMS3菌株进行发酵,用发酵液浇灌绿豆,观察其对绿豆各生长指标的影响。[结果]DMS3菌株16S rDNA大约1 500 bp,该菌有较高的解钾能力,发酵液中K+浓度达到1 020.646μg/L,用该菌液浇灌绿豆可明显提高株高、根长、鲜重、干重等指标。[结论]硅酸盐细菌在液体培养过程中能够产生某些促进植物生长的物质,同时能提高绿豆的抗旱能力,为该菌肥工业化开发应用奠定了基础。

[ Objective ] The research aimed to study the K-releasing effect of silicate bacteria provide scientific basis for producing bio-potasslum fertilizers by using the bacteria. [ Method] Silicate bacteria were selected from different regions. DMS3 was higher efficient strain and its physiological and biochemical characterization was identified. 16S rDNA gene of the bacteria was obtained by PCR amplification. Mung bean fed with fermentation of DMS3 was used to observe the impact on the growth indicators. [ Result] 16S rDNA gene of the bacteria was about 1 500 bp. DMS3 fermentation had a high potassium concentration, reached K ion concentration 1 020. 646 μg/L. The fermentation could significantly increase the plant height, root length, fresh weight, dry weight of mung bean. [ Conclusion ] Silicate bacteria in liquid culture can produce some substances to promote the growth of mung bean, at the same time to increase the capacity of drought. The research laid the foun- dation for the industrialization development and application of fertilizer.