火鸡组织滴虫检测方法研究进展

Progress on detection of Histomonas meleagridis in turkeys

火鸡组织滴虫病是由火鸡组织滴虫引起的禽类盲肠和肝脏机能紊乱的一种急性原虫病。常规的组织滴虫诊断方法有光学显微镜法、血清学检测法等，但这些方法存在费时、灵敏度不高、特异性不强等缺点，容易出现漏检、错检现象。分子生物学、免疫组织化学和酶联免疫吸附试验等方法为组织滴虫病的临床诊断提供了快速、高效的检测方法，可为组织滴虫病的流行病学研究提供重要的参考依据。

Histomoniasis is an acute primary disease causing the function disorder of turkey caecum and liver by the Histomonas meleagridis. Traditional detection methods such as optical microscopy, serology method for the diagnosis of this disease are time-consuming, of low sensitivity and specificity, easily miss-or fault-diagnosed. Molecular technology provides a more rapid and highly efficient detective method, while the immunohistochemical technique and ELISA can be used to study the sequential pathogenesis of histomonosis in birds and to obtain new insights into the mechanisms of interaction with the infected tissues.