搜风祛痰中药对AngⅡ诱导人脐静脉内皮细胞LOX-1 mRNA及NF-κB mRNA表达的影响

Effects of Expelling Wind and Expectorant Chinese Medicine on Expression of LOX-1 mRNA and NF-κ B mRNA Induced by AngiotensinⅡ in Human Umbilical Vein Endothelial Cells

目的观察搜风祛痰中药对AngⅡ诱导体外培养人脐静脉内皮细胞凝素样氧化低密度脂蛋白受体1(LOX-1)与核因子-κB基因表达的影响。方法制备家兔中药复方活心汤含药血清。采用酶消化法体外培养HUVECs2～3代用于实验。采用RT-PCR法测定脐静脉血管内皮细胞LOX-1 mRNA水平。随机分为正常对照组、AngⅡ组、AngⅡ+低剂量中药血清组、AngⅡ+中剂量中药血清组、AngⅡ+高剂量中药血清组。用中药先孵育2h后再加入0.5μmol/LAngⅡ刺激2h,取出细胞盖玻片固定。结果与对照组比较,活心汤含药血清可抑制AngⅡ导致的细胞损伤,减少LOX-1mRNA及NF-κB mRNA的表达。结论活心汤可抑制AngⅡ所致的HUVECs损伤,保护血管内皮细胞功能。

Objective： To observe the effect of expelling wind and expectorant Chinese medicine on the expression of LOX-1 mRNA and NF-κB mRNA induced by angiotensin Ⅱ（Ang Ⅱ） in human umbilical vein endothelial cells （HUVECs） . Methods： The serum containing metabolic ingredients of Huoxin Decoction was taken from rabbits. HUVECs 2-3 were cultured in vitro by enzyme digestion. LOX-1 mRNA was determined by RT-PCR. HUVECs were randomly divided into five groups： normal control group, Ang Ⅱ group, Ang Ⅱ low dosage drug serum group, Ang Ⅱ middle dosage drug serum group and Ang Ⅱ high dosage drug serum group. HUVECs were preincubated by Chinese medicine for 2h,and then were added Ang Ⅱ 0.5μmol/L to stimulate for 2h. At last,the cells were taken out to fix by coverslip. Results： Compared with control group, Huoxin Decoction drug serum could inhibit the injury of endothelial cells and decrease the expression of LOX-1 mRNA and NF-κB mRNA. Conclusion： Huoxin Decoction can inhibit the injury of HUVECs induced by Ang Ⅱ and protect vascular endothelial cells function.