生骨注射液对体外培养大鼠成骨细胞增殖和分化影响的实验研究

Experimental Study on Effects of Shenggu Injection on Proliferation and Differentiation of Rat Osteoblasts in Vitro

目的探讨生骨注射液对体外培养的大鼠成骨细胞增殖和分化的影响,及探讨生骨注射液促进骨折愈合的细胞机制。方法体外培养大鼠成骨细胞,应用MTT法、对硝基苯磷酸盐法观察生骨注射液对体外培养成骨细胞的增殖、ALP表达的影响。结果生骨注射液对成骨细胞具有明显的促增殖、分化作用。结论生骨注射液具有刺激体外培养成骨细胞增殖、分化的功能,这可能是生骨注射液促进骨折愈合的机制之一。

Objective： To study the effect of Shenggu lnjection（SGI） on proliferation and differentiation of rat osteoblasts in vitro and to explore its possible cellular mechanisms in promoting the fracture cicatrization. Methods： The rat osteoblasts were cultured in vitro according to the protocol. The MTT and the PNPP methods were used to observe the proliferation and expression of ALP of osteoblasts cultured in vitro stimulated by SGI. Results： It was found that SGI could promote the proliferation and differentiation of rat osteoblasts cultured in vitro. Conclusion： SGI can stimulate the proliferation and differentiation of rat osteoblasts cultured in vitro. This may be one of the mechanisms of SGI in promoting the fracture cicatrization.