松萝酸原料及自微乳的抑菌抗炎实验初步研究

Experimental Study on Bacteria Inhibiting and Anti-inflammation Effects of Usnic Acid Material and SMEDDS

目的考察松萝酸原料及自微乳的抗菌抗炎作用。方法采用体外抑菌实验观察松萝酸原料及自微乳的抑菌效果;采用MTT法考察松萝酸原料及自微乳制剂对腹腔巨噬细胞的细胞毒作用,及对热灭活大肠杆菌诱导小鼠腹腔巨噬细胞释放TNF-α的影响。结果松萝酸原料药及其自微乳制剂对金黄色葡萄球菌和肺炎球菌的生长有抑制作用,对绿脓杆菌和大肠杆菌无效。抗炎实验表明,松萝酸原料及自微乳在1～100μg/ml质量浓度范围内对小鼠腹腔巨噬细胞无显著细胞毒作用;不同质量浓度的原料药和自微乳制剂均可使热灭活大肠杆菌诱导的TNF-α水平有不同程度的下降,并呈一定的量-效关系。结论松萝酸原料药和自微乳制剂均有一定抗菌作用,并显示出较强抗炎效应,其中制剂作用更为明显,抗炎效应明确。

Objective： To observe the effects of usnic acid material and SMEDDS in bacteria inhibiting and anti-inflammation. Methods： The bacteria inhibiting experiment in vitro was used to observe the bacteria inhibiting effects of usnic acid material and SMEDDS. The cytotoxic action on peritoneal macrophage and the mouse peritoneal macrophage releasing TNF-α induced by thermal inactivation bacillus coli of usnic acid material and SMEDDS were determined by MTT. Results： The antibacterials experiment showed that the usnic acid material and the SMEDDS had inhibitory action to staphylococcus aureus and pneumococcus, but no inhibitory action to pseudomonas aeruginosa and bacillus coli. The anti-inflammation test showed that within the concentration of 1-100μg/ml both usnic acid material and SMEDDS had no cytotoxic action. The usnic acid material and SMEDDS with different dosage could bring the levels of the mouse peritoneal macrophage releasing TNF-α induced by thermal inactivation bacillus coli down, and it showed a dose-dependent relationship. Conclusion： Both usnic acid material and SMEDDS had certain antibacterials activity and good anti-inflammatory activity. The anti-inflammatory activity of SMEDDS is better than material.