不同pH值缓冲液处理下蚕豆叶片相对含水量、脯氨酸及丙二醛含量的变化

Change of relative water content,Proline content and Malondialdehyde content of Vicia faba leaves under different pH buffer treatments

研究了外源pH缓冲液处理下,离体和整株蚕豆叶片相对含水量、脯氨酸含量和丙二醛含量的变化。结果表明,酸碱处理离体蚕豆叶片10 min和30 min后,叶片组织相对含水量在pH 6.5时最高;酸性处理下的叶片脯氨酸含量较高;pH 5.0时丙二醛含量较高。整株蚕豆叶片处理5 h的叶片组织相对含水量在pH 6.5时最高,处理12 h以pH 5.0时最高;pH 5.5处理5 h叶片脯氨酸含量最高,处理12 h脯氨酸含量的变化不大;处理5 h时丙二醛含量的变化随pH的降低略有下降,但处理12 h丙二醛含量的变化不大。

To further elucidate the mechanism of xylem pH as a root signal under drought stress,a study was conducted on relative water content,Proline content and Malondialdehyde content of Vicia faba leaves under different pH buffer treatments.The results showed that for leaves in vitro,relative water content was the highest under pH 6.5;Pro content was higher under acidic treatment;and Malondialdehyde content was the highest under pH 5.0 treatment.For the whole plant treatment,relative water content was the highest under 5 h pH 6.5 treatment,or 12 h under pH 5.0 treatment;Pro content was the highest under 5 h pH 5.5 treatment,but little change under 12 h treatment;Malondialdehyde content decreased a little under 5 h treatment,or did not change under 12 h treatment.