含ACC脱氨酶菌株Rs-5对棉苗生长及其耐盐性的影响

Effect of strain Rs-5 containing ACC deaminase on growth and salt-tolerance of cotton seedling under salt stress

试验设置不同盐及不同Rs-5菌液浓度、不同菌液处理方式,通过盆栽试验,比较各种处理条件下棉苗的生长量和丙二醛、脯氨酸、可溶性糖含量等生理指标的变化。结果表明,Rs-5具有在盐胁迫条件下促进棉花幼苗生长并增加其耐盐性的作用,这种作用随盐浓度升高而增加,当盐浓度在0.4%以上时,菌株Rs-5对棉苗生长量和耐盐性影响均达显著水平(P<0.05),0.8%的盐浓度下均达极显著水平(P<0.01);同时Rs-5菌悬液处理效果最佳的浓度是OD600nm=0.3(约108U/ml),棉花的株高、根长和干重分别比无菌对照增加了8.9%,10.1%,17.5%,丙二醛含量降低了42.8%,脯氨酸、可溶性糖和可溶性蛋白分别增加17.9%,0.8%,151.4%。

In order to elucidate the effect of strain Rs-5 containing ACC deaminase on growth and salt-tolerance of cotton seedling under salt stress,the growth biomass of cotton and four physiological indices（MDA,proline,soluble sugar and soluble protein content） of the cotton seedling were studied by pot experiment under different salt concentrations,different Rs-5 bacteria suspension concentrations and in different treatment methods respectively.The results showed that cotton growth and its salt-tolerance under salt stress were promoted by strain Rs-5,and this effect was more obviously as salt stress increased.When salt concentration is above 0.4%,the influence of Rs-5 to both cotton growth and salt-tolerance reached a significant level（P0.05）,and reached a very significant level（P0.01） at a salt concentration 0.8%.Compared with control,the average plant height,root length,dry weight of cotton seedlings increased by 8.9%,10.1% and 17.5%,and proline,soluble sugar and soluble protein content increased by 17.9%,0.8% and 151.4% respectively,while MDA content decreased by 42.8%,and the stress relief and growth promotion of Rs-5 efficacy is the best,when the bacteria suspension concentration reached OD=0.3（about 108 U/ml）.