马来丝虫基因中长散状分布的同系核酸因子的转录和免疫学研究

Translation of LINE Homologues in the Filarial Parasite Brugia Malayi and Immunoreaction

呈长散状发布的核酸因子（LINE）是哺乳类动物基因中的复制DNAs。该DNA系列可高度转录，但IJNE的转录产物目前仅可被某些恶性肿瘤的细胞腺所识别。本文描述了从马来丝虫RNA筛选的以POLY-A诱导的三个CDNA克隆。马来丝虫RNA含有一个明显插入的与LINE第二个开放阅读结构（ORF）同系序系列及对病毒的逆转录酸。用其中一种丝虫CDNA克隆编码，并在细菌载体上表达，纯化的融合蛋白免疫白兔，可刺激其免疫系统产生特异性抗体。这种特异抗体不仅与逆转录酶发生免疫应答反应，同样也与马来丝虫提取物抗原反应。

Long Intersperwed Nuclear Elements (LINEs) are repetitiveDNAS in mammalian genomes. LINE sequences are highly tran-scribed. but LINE encoded translational products have only been iden tified so far in some malignant cell lines. Here we describe the chancediscovery that three cDNA clones derived from poly ~ A selected RNA from the filarial parasite Brugia malayi contain inserts with a striking sequence homology to the second ORF of LINE and to viral reverse transcriptases. Rabbit antisera raised againest the bacterially expressed fusion protein encoded by one of the filarial cDNA clones react not onlywith reverse transcriptase, but also with native molecules in parasite extracts and the microfilarial sheath.