基于UV光谱扫描天然彩色棉色素定量测定方法研究

Quantitative Analysis on Natural Colored Cotton Pigment Based on UV Spectral Scanning

【目的】测定彩色棉色素含量。【方法】对不同浓度天然彩色棉纤维色素粗提液扫描分析。【结果】绿色棉纤维色素提取液在252 nm附近吸收峰随色素浓度减小向短波长方向小幅度移动,棕色棉纤维色素吸收峰随浓度减小向紫外短波长方向移动幅度较大。对色素粗提液200~700 nm所有吸收值与相对浓度的相关系数进行筛选,选择最大相关系数对应波长为定量分析特征波长,确定绿色棉纤维提取液定量分析特征波长为417 nm,棕色棉纤维提取液定量分析特征波长为613 nm,并且建立直线回归方程,回归方程分别为：C绿=0.0535A,R2=0.9965;C棕=0.0217A,R2=0.9878。相关系数均达到0.99以上。【结论】彩色棉纤维色素吸收值对相对浓度的回归方程有明确意义。

【Objective】Quantitative Analysis of color plays major role in inheritance and breeding of naturally colored cotton.【Method】In present study,pigment of color cotton fibers was extracted and the absorbance was measured using scanning UV spectrum.【Result】It is observed the absorbance peak of the green color fiber pigment extraction solution occurred at 252 nm,and the absorbance peak moved heading to direction of shortwave gradually with decreasing the concentration of green color pigment,while the absorbance peak of brown color fiber pigment extraction solution moved more obvious heading to the direction of ultraviolet region shortwave,compared with green color pigment.The quantitative relationship between absorbance and the concentration of different color fibers pigment was calculated and the highest correlation coefficient was screened from all absorbance values（200-700 nm）,it is concluded that 417 nm and 613 nm were considered as characteristic wavelength for green color fiber and brown color fiber respectively.The regression equation between pigment concentration and absorbance were Cgreen=0.053 5 A（R2=0.996 5） for green color fiber and Cbrown=0.021 7 A（R2=0.987 8） for brown color fiber respectively.From this study a conclusion can be drawn that scanning UV spectrum method is a very powerful tool to determine the pigment content of color fiber.【Conclusion】Therefore,this method has very important significance for colored cotton breed.