生脉注射液对急性百草枯中毒大鼠肝损伤NO和iNOS的影响

Effects of Pulse-activating Injection on NO and iNOS in Rats with Acute Hepatic Injury Induced by Paraquat

目的:观察百草枯(PQ)急性中毒大鼠所致肝损伤时一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的变化,探讨生脉注射液对急性百草枯中毒所致肝损伤的保护作用。方法:将50只SD大鼠随机分成5组,空白组、阴性对照组、阳性对照组、生脉低剂量组和生脉高剂量组。观察大体标本,组织病理,测定血清中ALT、AST、MDA、SOD和GSH-Px。同时测定肺组织NO含量和iNOS活性。结果:与阴性对照组相比,生脉低剂量组肝组织病理显示肝出血明显减轻。ALT、AST、MDA、SOD和GSH-Px变化均有统计学意义(P<0.05,P<0.01),NO和iNOS也降低(P<0.01)。结论:NO及iNOS在百草枯所致大鼠肝损伤中起一定作用,生脉注射液能降低NO及iNOS水平,减轻百草枯中毒大鼠肝组织损伤。

Objective： The variance of NO and iNOS on Paraquat induced rat acute hepatic injury was investigated, and the protection of pulse-activating injection on rat acute hepatic injury was approached. Methods： 50 Sprague- Dawley （SD） rats were randomly divided into five experimental groups： blank group, negative control group, positive control group, pulse-activating injection low-dose group（LDG）, pulseactivating injection high-dose group（HDG）. Microscopical histopathologieal examinations were performed. In the mean time, the level of ALT, AST, MDA, SOD and GSH-Px in plasma were detected, and the activities of liver tissue homogenate NO and iNOS were measured. Results： Compared with that in negativecontrol group,hemorrhage of liver of pulse-activating injection low-dose group were mitigated. ALT、 AST、 MDA、 SOD and GSH-Px in plasma have statistical significance （P〈0.05, P〈0.01 ） ; and biological indexes of hepatic injury and the contents of NO and iNOS were significantly decreased （P〈0.05, P〈0.01 ）. Conclusions： NO and iNOS could play an role in hepatic injury of poisoned rats, pulse-activating injection could ameliorate the hepatic injury and decrease the concentrations of NO and iNOS.