高效液相色谱法测定盐酸麻黄碱滴鼻液的含量

Determination of Ephedrine Hydrochloride in Ephedrine Hydrochloride Nasal Drops by HPLC

目的建立用高效液相色谱（HPLc）法测定盐酸麻黄碱滴鼻液中盐酸麻黄碱含量的方法。方法选用Diamonsil（钻石）C18（5μm，150mm×4．6mm）为分析柱；检测波长为215nm；柱温为35℃；甲醇-0．01mol／L磷酸二氢钾（20：80）为流动相；流速为0．8ml／min。结果盐酸麻黄碱的保留时间约为7．9min，在43．776—136．800txg／ml的浓度范围内线性关系良好（r=0．9999）。平均回收率为100．4％，相对标准偏差值（RSD）为0．59％；供试品溶液在18h内稳定。结论该方法精密度好，准确度高，简单易行。

Objective To develop a method for determination of Ephedrine Hydrochloride in Ephedrine Hydrochloride nasal drops by HPLC. Methods The HPLC system was consisted of a Diamonsil （diamond） C18 （5 μm, 150 mm ×4.6 mm） at 35℃, and the UV-vis detective wave length was 215 mm. The mobile phase was composed of Methanol-0. 01 mol/L Potassium Dihydrogen Phosphate （20： 80）, and the flowing rate was 0.8 mL/min. Results The retention time of Ephed- rine Hydrochloride was about 7.9 min. Ephedrine Hydrochloride had good linearity within the range of 43. 776 - 136. 800 μg/ml（r =0. 9999）. The average recovery was 100.4% , and RSD was 0.59%. Sample solution was stable within 18 h. Conclusion The developed method is simple, fast, and sensitive, thus suitable for the analysis of Ephedrine Hydrochloride in Ephedrine Hydrochloride nasal drops.