摘要
南方菜豆花叶病毒(SBMV)主要有2株系:菜豆株系(SBMV-B)和豇豆株系(SBMV-C),血清学方法不能予以区分。根据已报道的核酸序列设计了2对特异引物,进行RT-PCR扩增并克隆到载体Bluescript中,序列测定予以证实后利用随机引物法合成放射性cDNA探针,再进行体外转译合成地高辛UTP标记的RNA探针。2种探针均可分别特异地检测Northernblot膜上的SBMV-B和SBMV-C。RNA探针检测SBMV-B和SBMV-C灵敏度分别为:0.1μg和0.01μg。
As serological techniques could not distinguish SBMV B and SBMV C,two type strains of southern bean mosaic virus,the fragments of the nucleic acid of the two strains were cloned and the specific radioactive cDNA and digoxigenin UTP labeled RNA probes were prepared.Both of the probes could specifically detect SBMV B SBMV C respectively on northern blot,as little as 0.1μg of SBMV B and 0.01μg of SBMV C were visualized.
出处
《植物病理学报》
CAS
CSCD
北大核心
1998年第3期243-248,共6页
Acta Phytopathologica Sinica