皮下移植和皮下注射构建子宫内膜异位症皮下模型的比较研究

Comparison between two experimental methods of reproduction of subcutaneous endometriosis model in nude mice

目的比较皮下移植和皮下注射两种方法建立裸鼠人子宫内膜异位症模型的效果及血管新生相关因子表达的差异。方法雌性BALB/c裸鼠41只,随机分为皮下移植组(A组,n=22)和皮下注射组(B组,n=19),分别采用皮下移植人子宫内膜块和皮下注射人子宫内膜碎屑的方式建模。每组又分为2、4周两个亚组,即A1、A2组和B1、B2组。采用SP法检测异位病灶血管内皮生长因子(VEGF)表达和微血管密度(MVD),并比较组间的异位病灶的体积和重量。结果在相同时间点(2周或4周),两组异位病灶体积差异无统计学意义(P>0.05),A组异位病灶重量较B组明显增加(P<0.05),而两组病灶腺体和间质中VEGF表达、MVD差异无统计学意义(P>0.05)。两组在2周时病灶腺体中VEGF表达及MVD均明显高于4周时(P<0.05)。结论两种方法均可成功构建裸鼠人子宫内膜异位症模型,并可观察血管新生情况,以皮下移植造模效果较好。

Objective To compare the results of two experimental methods, namely subcutaneous transplant and subcutaneous injection, in reproducing endometriosis in nude mice, and study the differences in angiogenesis related factors. Methods Nude mice were divided two groups： in group A, the human endometrium fragments were transplanted into subcutaneously, and in group B the human endometrium suspension was subcutaneously injected. Each group was then divided into two subgroups according to inplantation time （2 and 4 weeks）： group A1 and group A2, and group B1 and group 132. Vascular endothelial growth factor （VEGF） and microvessel density （MVD） were detected by SP method, and the weight and volume of ectopic focus between different groups were compared. Results At the same time points （2 week or 4 week）, the volume of ectopie focus showed no significant difference between group A and group B （P〉 0. 05）, but the weight of ectopic focus was significantly higher in group A than in group B （P〈0. 05）. There was no difference in VEGF expression and MVD in glandular cells and intercellular substance between group A and group B （P〉0. 05）, but the VEGF expression and MVD in gland cells were significantly higher in A1 and B1 groups at 2 weeks compared with that at 4-week point in A2 and B2 groups （P〈0. 05）. Conclusion It is successful in reproducing human endometriosis model in nude mice with both methods. Trasplantation method may be detter for observation of angiogenesis.