湖南地区汉族人群中HLA-A、B、DRB1等位基因与原因不明习惯性流产相关性研究

Association of HLA-A,-B and-DRB1 alleles with unexplained spontaneous abortions in Han population of Hunan province,China

探讨HLA-A、-B和-DRB1位点等位基因多态性与原因不明习惯性流产的相关性。应用PCR-SSP方法对175对(350例)湖南汉族原因不明习惯性流产(URSA)患者夫妇进行HLA-A、-B、-DRB1基因分型,并以2 000例(男女各1 000例)湖南无关健康汉族个体造血干细胞分型资料作为对照,分析HLA等位基因频率在两组中的分布差异。结果是HLA-A、-B及-DRB1位点各等位基因频率在对照组男女中均无显著差异(P>0.05);URSA患者组A*31(2.86%)、B*08(0.86%)、B*39(4.14%)和B*56(1.86%)的等位基因频率显著高于对照组(RR值分别为2.07、2.99、1.56和2.19;P值分别为0.0059、0.0276、0.0404和0.0172);在URSA妻子组中,B*39(4.57%)显著高于对照组(RR=1.75;P=0.0474),A*33(3.71%)、B*58(3.14%)和DRB1*03(2.29%)的等位基因频率明显低于对照组(RR值分别为0.57、0.51和0.50;P值分别为0.0339、0.0182和0.0383);A*31(3.14%)、B*08(1.43%)、B*52(2.29%)及B*56(2.29%)在URSA丈夫组中的等位基因频率均高于对照组(RR值分别为2.32、5.08、2.31和2.77;P值分别为0.013、0.0012、0.0374及0.0105)。结果表明湖南汉族人群中A*31、B*08、B*39、B*52和B*56可能是URSA的易感等位基因,而A*33、B*58和DRB1*03可能对URSA的发生有保护作用。

To investigate the association between the HLA-A, B and -DRB1 polymorphism and the unexplained recurrent spon taneous abortions （URSA）, the HLA-A, -13 and - DR131 genotyping of 175 couples （350 individuals） with URSA were inves tigated by polymerase chain reaction sequence specific primers （PCR SSP） method. The allele frequencies （AFs） of HLA-A,B and -DRB1 of the patients were calculated and compared with the control group （2 000 normal health individuals, 1 000 male and 1 000 female） using a chisquare test. h was found that there was no significantly difference between the male and female AFs in the control group. The AFs of A ＊ 31（2.86%）, B ＊ 08（0.86 %）, B ＊ 39（4.14%） and B ＊ 56（1.86%） were significant ly higher in the URSA patients than those in controls （RR=2.07, 2.99, 1.56, 2. 19; P=0. 0059, 0. 0276, 0. 0404, 0. 0172 respectively）. When the patients group was divided into wife group and husband group, B ＊ 39 （AF= 4.57%, RR= 1.75,P= 0. 0474 ） was significantly increased in the URSA wife group, while A ＊ 33 （3.71 % ）, B ＊ 58 （3.14% ） and DRB1 ＊ 03 （2.29 % ） were significantly decreased （RR=0.57, 0. 51, 0. 50; P = 0. 0339, 0. 0182, 0. 0383 respectively）. The AFs of A ＊ 31 （3. 14 % ）, B ＊ 08 （1.43 % ）, B ＊ 52 （2.29 % ） and B ＊ 56 （2.29 % ） in the URSA husband group were strikingly higher than those in controls （RR=2.32, 5.08, 2.31, 2.77;P=0. 013, 0. 0012, 0. 0374, 0. 0105 respectively）. Our data suggest that HLA-A 31, B ＊ 08, B＊ 39, B＊ 52 and B＊ 56 may contribute to the genetic susceptibility to URSA, while A＊ 33, B＊ 58 and DRB1 ＊03 may play the protective role.