系统性红斑狼疮患者CD8^+T细胞亚群Fas-FasL的表达及凋亡的关系研究

Relationship between expressions of Fas-FasL and apoptosis of peripheral blood CD8^+T lymphocytes in patients with systemic lupus erythematosus

为检测系统性红斑狼疮患者外周血CD8+CD28-T细胞Fas-Fasl、CD8+CD28+T细胞Fas-Fasl的表达情况及与凋亡的关系。采用三抗体标记流式细胞术(FCM)检测SLE患者活动期(22例)及稳定期(18例)外周血CD8+CD28-T细胞Fas-Fasl、CD8+CD28+T细胞Fas-Fasl表达率及凋亡率。结果是(1)SLE活动组患者CD8+CD28-T细胞亚群Fas表达率较稳定组及正常组显著增高(P<0.05);SLE患者CD8+CD28+T细胞亚群Fas表达率呈活动组>稳定组>正常组的特点,组间有显著统计学差异(P<0.05),FasL表达率活动组患者较稳定组及正常组显著增高(P<0.05);CD8+T细胞凋亡率在活动组患者较稳定组及正常组显著增高(P<0.05);(2)SLE活动性与CD8+T细胞凋亡率呈正相关(P<0.01),与CD8+CD28-及CD8+CD28+T细胞亚群Fas、FasL表达率间无相关关系;(3)CD8+CD28+T细胞亚群Fas与FasL表达与凋亡率有相关性且该细胞亚群Fas表达与FasL表达有明显相关性,而CD8+CD28-T细胞亚群Fas表达与FasL表达无明显相关性。提示SLE患者外周血CD8+T细胞凋亡率异常增高,SLE活动性与CD8+T细胞凋亡率呈正相关,活动期与稳定期Fas-Fasl的表达差异可能导致了免疫耐受机制障碍。

To investigate the relationship between expression of Fas-FasL and apoptosis of peripheral blood CD8^＋ CD28^＋ and CD8^＋ CD28^＋ T lymphocytes in patients with systemic lupus erythematosus（SLE）, the rates of Fas-FasL expression and apoptosis of CD8^＋ CD28^＋ and CD8^＋ CD28^＋ T cells were measured by three antibodies labeling flow cytometry（FCM） analysis in 22 active cases, 18 inactive cases of SLE and 20 controls. The results showed that： （1）Compared to controls and inactive SLE, apoptosis rates of CD8^- T cells was significantly higher in active SLE group（P〈0.05）, and the expression of Fas in CD8^＋ CD28^＋ T cells and FasL in CD8^＋ CD28^＋ T ceils were higher in active SLE group（P〈0.05）. The expression rate of Fas of CD8^＋ CD28^＋ T cells in SLE patients was featured by the fact that active SLE group 〉 inactive SLE group〉control group, and there was a significant difference in statistics in these 3 groups（P〈0.05）. （2） The activity of SLE was positively correlated with the apoptosis rate of CD8^- T cells, but there was no correlation with expression of Fas.FasL in CD8 ^＋ CD28^＋ and CD8^＋ CD28^＋ T cells. （3） The expression of Fas,FasL were correlated with apoptosis,and expression of Fas was significant correlated with expression of FasL in CD8^＋ CD28^＋ T cells. The expression of Fas was not significantly correlated with expression of FasL in CD8^＋ CD28^＋ Tcells. It is concluded that the apoptotic rate of the peripheral blood CD8^＋ T cells is abnormally increased and the activity of SLE was positively correlated with the apoptotic rate of CD8^＋ T cells. The difference of expression in Fas and FasL between active and inactive cases of SLE may induce the disability of immune tolerance.