n-3多不饱和脂肪酸对大鼠小肠移植慢性排斥肠黏膜细胞凋亡的抑制作用

n-3 PUFA can inhibit the apoptosis of intestinal epithelial cells of chronic rejection after small intestinal transplantation

目的:探讨大鼠小肠移植后移植肠发生慢性排斥时,口服n-3多不饱和脂肪酸对移植肠黏膜细胞凋亡、颗粒酶B和穿孔素表达的调控作用。方法:将大鼠分为三组:①Lewis-Lewis组,口服玉米油;②F344-Lewis组,口服玉米油;③F344-Lewis组,口服鱼油。所有受鼠在移植术后16周取材检测。用流式细胞法检测肠黏膜细胞凋亡,RT-PCR法检测穿孔素和颗粒酶的表达。结果:慢性排斥发生后,移植肠黏膜细胞凋亡率、颗粒酶B和穿孔素的表达明显增加。口服鱼油可抑制移植肠黏膜细胞的凋亡、颗粒酶B和穿孔素的表达。讨论:口服n-3多不饱和脂肪酸能抑制慢性排斥期移植肠的排斥反应,提高受鼠和移植物的存活率,延缓移植物的慢性失功。

Objective：The aim of our work was to investigate the effects of n-3 polyunsaturated fatty acids on apoptosis, granzyme B and perforin expression of intestinal epithelial cells of chronic rejection after small intestinal transplantation. Methods： Small bowel transplantation was performed and rats were divided into three groups ： Group 1, Lewis-to-Lewis, group 2, F344-to-Lewis, dietary corn oil, Group 3, F344-to-Lewis, dietary fish oil. All recipients were killed after 16 weeks of posttransplantation. The apoptosis rate of mucosal cells were evaluated by flow cytometry. The expressions of granzyme B and perforin were analyzed by reverse transcriptase RT-PCR. Results： A high apoptotic rate was observed when the allografts demonstrated one or more histological features of chronic rejection. N-3 polyunsaturated fatty acids decreased the rate of the apoptosis and inhibitted the expressions of granzyme B and perforin. Conclusion： N-3 polyunsaturated fatty acids can suppress the chronic rejection in small intestinal transplantation.