摘要
目的探讨端粒酶、人端粒酶RNA组分(hTR)基因表达、分布及其与人端粒酶蛋白基因编码催化蛋白亚基(hTERT)基因表达的关联性。方法对51例宫颈活检组织进行端粒酶hTR、hTERT基因原位杂交检测。结果51例组织中28例hTR原位杂交阳性,32例hTERT阳性,表达随着病变的严重程度而增加。在高度鳞状上皮内病变(HSIL)/宫颈鳞状上皮癌(SCC)和低度鳞状上皮内病变(LSIL)/炎症组间差异有统计学意义,而且hTR和hTERT表达具有相关性(P<0.05)。结论在宫颈病变组织中,检测hTR基因表达情况可用于宫颈癌的早期诊断和鉴别。
Objective To explore the expression and distribution of hTR gene and its relationship with hTERT mRNA expression in human uterine cervical tissues. Methods 51 cervical samples were divided into 4 groups （cervieitis, LSIL, HSIL and SCC） and detected with ISH of hTR and hTERT RNA. Results There were 28 and 32 positive expression respectively in 51 samples, and their expression increasing with cervical lesion severe degrees. The difference is significant between HSIL/SCC and LSIL/Cervieitis groups, and the correlation was also significant with hTR and hTERT gene expression （P〈0.05）. Conclusion The detection of hTR mRNA expres- sion may be used to diagnose and differentiate in early cervical carcinoma.
出处
《山西医药杂志（上半月）》
CAS
2009年第12期1081-1083,共3页
Shanxi Medical Journal
基金
深圳市科技计划项目(200204068
200802033)
