摘要
在昆明地区栽培的香石竹上发现了香石竹斑驳病毒,人工接种可局部侵染苋色藜、墙生藜、千日红及番杏,可系统侵染昆诺阿藜和美国石竹,分别造成褪绿斑、斑驳或坏死斑。经二轮PEG(分子量6000)沉淀,病毒提纯量约220mg/kg鲜组织。电镜观察病毒颗粒直径为28nm,等轴对称。提纯物紫外扫描呈典型病毒核蛋白吸收峰,A260/A280值为1.56。提纯病毒经4次免疫家兔,所得抗血清经试管沉淀测定效价为1∶4096,琼脂糖双扩散效价为1∶1024。
Carnation mottle virus was found from carnation plants grown in Kunming District. It could locally infected Chenopodium amaranticolor, C. murale, Gomphrena globosa, Tetragonia expansa, and systemically infected C. quinoa, Dianthus barbatus, and induced chlorotic lesion, mottle or necrotic spots on these hosts respectively. By two cycles of PEG (MW 6000) precipitation, the virus yield was about 220 mg/kg in fresh tissues. Diameter of the isometric particle was 28 nm. With UV absorption spectrum scaning, the ratio of A 260 /A 280 was 1 56. The titre of antiserum was 1∶4 096 in convention tube precipitation test and 1∶1 024 in gel double diffusion test.
出处
《植物保护》
CAS
CSCD
北大核心
1998年第5期5-7,共3页
Plant Protection
关键词
香石竹
斑驳病毒
鉴定
提纯
抗血清
carnation mottle virus, identification, PEG precipitation, antiserum preparation