HPLC测定贝诺酯片的含量

Determination of Benorilate tablets by HPLC

目的采用HPLC法测定贝诺酯片的含量。方法色谱柱为C18柱,流动相为甲醇-水(56:44,磷酸调pH3.5),检测波长为240nm。结果贝诺酯进样量0.52～6.24μg与峰面积的线性关系良好(r=0.9999),进样精密度、重复性良好,平均回收率为101.1%。结论所用方法准确、简便,可有效地控制贝诺酯片的含量。

OBJECTIVE To establish an HPLC method to determine Benorilate tablets. METHODS A C18 column was used. The mobile phase consistsed of methanol and water（56 ： 44, pH adjusted to 3.5 with phosphoric acid）. The UV detector was set at 240nm. RESULTS The calibration curve showed good linearity over the range of 0.52 - 6.24μg（ r=0. 9999）, the average recovery rate was 101.1%. CONCLUSION The new method can be used to determine the contents of Benorilate tablets.