HPLC测定不同来源白鲜皮中黄柏酮含量

HPLC determination of obacunone in Cortex Dictamni in different areas

目的:研究不同地区白鲜皮药材及饮片中黄柏酮的含量。方法:采用高效液相色谱法,色谱柱为AgilentC18(150nm×4.6nm,5μm),流动相为甲醇-水(60∶40);检测波长212nm。结果:加样回收率为96.71%;RSD为1.56%;相关系数r=0.9999;不同地区的白鲜皮药材与白鲜皮饮片中,黄柏酮的含量范围为2.41～3.01mg/g。结论:不同地区白鲜皮药材与相应饮片中,黄柏酮含量基本相同。

Objective： To establish a HPLC method to determine the contents of Obacunone in Cortex Dictamni in diffe~ ent areas.Methods：Selected Agilent Cjs （150 nm×4.6 rim, 5 μm） ;The mobile phase was consisted of methanol-water （61 40）;the detection wavelength was at 212 nm. Results：The average recovery was 96.71%;RSD was 1.56%;r was 0.999 9.The contents of Obacunone in Cortex Dictamni in different areas were 2.41-3.01 mg/g.Conclusion ：Cortex Dictamni in differel areas contains similar amount of Obacunone. ｜